Differential Gene Expression in TRAP-positive Cells During Mouse Embryonic Skeletal Development

....................................................................Page 6 Introduction................................................................Pages 7-11 Materials and Methods...................................................Pages 12-17 Results......................................................................Pages 18-31 Discussion..................................................................Pages 32-41 Bibliography...............................................................Pages 42-44 FIGURES AND TABLES Figure 1: TRAP Stained Section from an E18.5 Femur Figure 2: Main Components of Laser Capture Microdissection Figure 3: LCM Picture of Before, During and After Capture Figure 4: Ct Expression Housekeeping Genes Figure 5: Expression of Known Markers for Osteoblasts and Hypertrophic Chondrocytes. Figure 6: Ct Expression for Candidate Genes Figure 7: Log Fold Expression Ratio for E18.5 Figure 8: Mean Relative Fold induction Figure 9: Remodeling of Mineralized Cartilage during Endochondral Ossification. Figure 10: Known Genetic Interactions for each of the Candidate Genes Figure 11: Model of Chondroclast and Osteoclast Lineage Table 1: Genes and their Specific Primers Table 2: Potential Endogenous Control Genes Table 3: Known Markers of Chondrocyte and Osteoblast Cells Table 4: Relative Quantitation of Col10A1 Expression in Chondroclasts and Hypertrophic Chondrocytes Table 5: Candidate Genes Table 6: Classification of the Genes